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dc.contributor.authorSinkala, Elly
dc.contributor.authorMcCutcheon, James E.
dc.contributor.authorSchuck, Matt
dc.contributor.authorSchmidt, Eric
dc.contributor.authorRoitman, Mitchell F.
dc.contributor.authorEddington, David T.
dc.date.accessioned2013-10-25T15:05:49Z
dc.date.available2013-12-06T22:55:21Z
dc.date.issued2012-07
dc.identifier.bibliographicCitationSinkala E, McCutcheon JE, Schuck MJ, Schmidt E, Roitman MF, Eddington DT. Electrode calibration with a microfluidic flow cell for fast-scan cyclic voltammetry. Lab on a Chip. 2012 Jul 7;12(13):2403-8. DOI: 10.1039/c2lc40168aen_US
dc.identifier.issn1473-0197
dc.identifier.urihttp://hdl.handle.net/10027/10318
dc.descriptionPost print version of article may differ from published version. The definitive version is available through Royal Society of Chemistry at DOI: 10.1039/c2lc40168aen_US
dc.description.abstractFast-scan cyclic voltammetry (FSCV) is a common analytical electrochemistry tool used to measure chemical species. It has recently been adapted for measurement of neurotransmitters such as dopamine in awake and behaving animals (in vivo). Electrode calibration is an essential step in FSCV to relate observed current to concentration of a chemical species. However, existing methods require multiple components, which reduce the ease of calibrations. To this end, a microfluidic flow cell (μFC) was developed as a simple device to switch between buffer and buffer with a known concentration of the analyte of interest – in this case dopamine - in a microfluidic Y-channel. The ability to quickly switch solutions yielded electrode calibrations with faster rise times and that were more stable at peak current values. The μFC reduced the number of external electrical components and produced linear calibrations over a range of concentrations. To demonstrate this, an electrode calibrated with the μFC was used in FSCV recordings from a rat during the delivery of food reward – a stimulus that reliably evokes a brief increase in current due to the oxidation of dopamine. Using the linear calibration, dopamine concentrations were determined from the current responses evoked during the behavioral task. The μFC is able to easily and quickly calibrate FSCV electrode responses to chemical species for both in vitro and in vivo experiments.en_US
dc.description.sponsorshipSupport for this work was provided by NIH MH085073 and NIH R01 DA025634 to MFRen_US
dc.language.isoen_USen_US
dc.publisherRoyal Society of Chemistryen_US
dc.subjectelectrodeen_US
dc.subjectcalibrationen_US
dc.subjectdopamineen_US
dc.subjectfast-scan cyclic voltammetryen_US
dc.subjecty-channelen_US
dc.titleElectrode Calibration with a Microfluidic Flow Cell for Fast-scan Cyclic Voltammetryen_US
dc.typeArticleen_US


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