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dc.contributor.authorEapen, Asha
dc.contributor.authorRamachandran, Amsaveni
dc.contributor.authorGeorge, Anne
dc.date.accessioned2013-11-01T17:25:52Z
dc.date.available2013-11-01T17:25:52Z
dc.date.issued2012-02-17
dc.identifier.bibliographicCitationEapen, A. S., Ramachandran, A., & George, A. 2011. Dentin phosphoprotein (DPP) activates integrin-mediated anchorage-dependent signals in undifferentiated mesenchymal cells. Journal of Biological Chemistry, 287(8): 5211-24. doi: 10.1074/jbc.M111.290080en_US
dc.identifier.issn0021-9258
dc.identifier.otherdoi: 10.1074/jbc.M111.290080
dc.identifier.urihttp://hdl.handle.net/10027/10395
dc.descriptionThis research was originally published in Journal of Biological Chemistry. Eapen A, Ramachandran A, George A.. Dentin phosphoprotein (DPP) activates integrin-mediated anchorage-dependent signals in undifferentiated mesenchymal cells. Journal of Biological Chemistry. 2012. 287(8):5211-24. © the American Society for Biochemistry and Molecular Biology.en_US
dc.description.abstractDPP, a major noncollagenous protein of the dentin matrix is a highly acidic protein and binds Ca2+ avidly and thus linked to matrix mineralization. Here, we demonstrate that the RGD domain in DPP can bind to integrins on the cell surface of undifferentiated mesenchymal stem cells and pulp cells. This coupling generates intracellular signals that are channeled along cytoskeletal filaments and activates the nonreceptor tyrosine kinase FAK, which plays a key role in signaling at sites of cellular adhesion. The putative FAK autophosphorylation site Tyr397 is phosphorylated during focal adhesion assembly induced by DPP on the substrate. We further demonstrate that these intracellular signals propagate through the cytoplasm and activate anchorage-dependent ERK signaling. Activated ERK, translocates to the nucleus and phosphorylates the transcription factor ELK-1which in turn coordinate the expression of downstream target genes such as DMP1 and DSP. These studies suggest a novel paradigm which demonstrates that extracellular DPP can induce intracellular signaling which can be propagated to the nucleus and thus alter gene activities.en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health Grant DE 19633 and the Brodie Endowment Fund.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biologyen_US
dc.subjectDPPen_US
dc.subjectIntegrinsen_US
dc.subjectFAKen_US
dc.subjectExtracellular matrixen_US
dc.subjectDifferentiationen_US
dc.subjectGene expressionen_US
dc.subjectDentinen_US
dc.titleDPP Activates Integrin-Mediated Anchorage-Dependent Signals in undifferentiated mesenchymal cellsen_US
dc.typeArticleen_US


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