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dc.contributor.authorMauleon, Gerardo
dc.contributor.authorFall, Christopher P.
dc.contributor.authorEddington, David T.
dc.date.accessioned2013-12-03T22:14:04Z
dc.date.available2013-12-03T22:14:04Z
dc.date.issued2012-08
dc.identifier.bibliographicCitationMauleon G, Fall CP, Eddington DT. Precise Spatial and Temporal Control of Oxygen within In Vitro Brain Slices via Microfluidic Gas Channels. PLoS One. 2012;7(8). DOI: 10.1371/journal.pone.0043309en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10027/10767
dc.descriptionThis is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. The original version is available through Public Library of Science at DOI: 10.1371/journal.pone.0043309.en_US
dc.description.abstractThe acute brain slice preparation is an excellent model for studying the details of how neurons and neuronal tissue respond to a variety of different physiological conditions. But open slice chambers ideal for electrophysiological and imaging access have not allowed the precise spatiotemporal control of oxygen in a way that might realistically model stroke conditions. To address this problem, we have developed a microfluidic add-on to a commercially available perfusion chamber that diffuses oxygen throughout a thin membrane and directly to the brain slice. A microchannel enables rapid and efficient control of oxygen and can be modified to allow different regions of the slice to experience different oxygen conditions. Using this novel device, we show that we can obtain a stable and homogeneous oxygen environment throughout the brain slice and rapidly alter the oxygen tension in a hippocampal slice. We also show that we can impose different oxygen tensions on different regions of the slice preparation and measure two independent responses, which is not easily obtainable with current techniques.en_US
dc.description.sponsorshipNational Institute of Mental Health Neurotechnology program through R21MH085073 to CPF and DTEen_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.titlePrecise Spatial and Temporal Control of Oxygen within In Vitro Brain Slices via Microfluidic Gas Channelsen_US
dc.typeArticleen_US


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