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dc.contributor.authorWu, Yangzhe
dc.contributor.authorYu, Tian
dc.contributor.authorGilbertson, Timothy A.
dc.contributor.authorZhou, Anhong
dc.contributor.authorXu, Hao
dc.contributor.authorNguyen, Kytai Truong
dc.date.accessioned2013-12-05T18:46:10Z
dc.date.available2013-12-05T18:46:10Z
dc.date.issued2012-05
dc.identifier.bibliographicCitationWu, Y. Z. Yu, T. Gilbertson, T. A. Zhou, A. H. Xu, H. Nguyen, K. T. (2012) Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells. PLoS ONE 7(5): e36885. doi:10.1371/journal.pone.0036885en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10027/10817
dc.description© 2012 Wu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.abstractExposure to diesel exhaust particles (DEPs), a major source of traffic-related air pollution, has become a serious health concern due to its adverse influences on human health including cardiovascular and respiratory disorders. To elucidate the relationship between biophysical properties (cell topography, cytoskeleton organizations, and cell mechanics) and functions of endothelial cells exposed to DEPs, atomic force microscope (AFM) was applied to analyze the toxic effects of DEPs on a model cell line from human aortic endothelial cells (HAECs). Fluorescence microscopy and flow cytometry were also applied to further explore DEP-induced cytotoxicity in HAECs. Results revealed that DEPs could negatively impair cell viability and alter membrane nanostructures and cytoskeleton components in a dosage- and a time-dependent manner; and analyses suggested that DEPs-induced hyperpolarization in HAECs appeared in a time-dependent manner, implying DEP treatment would lead to vasodilation, which could be supported by down-regulation of cell biophysical properties (e.g., cell elasticity). These findings are consistent with the conclusion that DEP exposure triggers important biochemical and biophysical changes that would negatively impact the pathological development of cardiovascular diseases. For example, DEP intervention would be one cause of vasodilation, which will expand understanding of biophysical aspects associated with DEP cytotoxicity in HAECs.en_US
dc.description.sponsorshipThis work is partially supported by Huntsman Environmental Research Center and Utah Water Research Laboratory, Logan, UT (to AZ). The authors also acknowledge the partial financial support from the National Institutes of Health grant HL091232 (to KTN).en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.titleBiophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cellsen_US
dc.typeArticleen_US
dc.identifier.citationWu, Y. Z. Yu, T. Gilbertson, T. A. Zhou, A. H. Xu, H. Nguyen, K. T. (2012) Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells. PLoS ONE 7(5): e36885. doi:10.1371/journal.pone.0036885


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