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dc.contributor.authorKotlo, Kumar
dc.contributor.authorBhattacharyya, Sumit
dc.contributor.authorYang, Bo
dc.contributor.authorFeferman, Leonid
dc.contributor.authorTejaskumar, Shah
dc.contributor.authorLinhardt, Robert
dc.contributor.authorDanziger, Robert
dc.contributor.authorTobacman, Joanne K.
dc.date.accessioned2013-12-05T19:59:34Z
dc.date.available2014-10-07T09:30:16Z
dc.date.issued2013-10
dc.identifier.bibliographicCitationKotlo K, Bhattacharyya S, Yang B, Feferman L, Tejaskumar S, Linhardt R, Danziger R, Tobacman JK. Impact of salt exposure on N-acetylgalactosamine-4-sulfatase (arylsulfatase B) activity, glycosaminoglycans, kininogen, and bradykinin. Glycoconjugate Journal. 2013 Feb 6. DOI: 10.1007/s10719-013-9468-8en_US
dc.identifier.issn1573-4986
dc.identifier.urihttp://hdl.handle.net/10027/10826
dc.descriptionPost print version of article may differ from published version. The final publication is available at springerlink.com; DOI: 10.1007/s10719-013-9468-8en_US
dc.description.abstractN-acetylgalactosamine-4-sulfatase (Arylsulfatase B; ARSB) is the enzyme that removes sulfate groups from the N-acetylgalactosamine-4-sulfate residue at the non-reducing end of chondroitin-4-sulfate (C4S) and dermatan sulfate (DS). Previous studies demonstrated reduction in cell-bound high molecular weight kininogen in normal rat kidney (NRK) epithelial cells when chondroitin-4-sulfate content was reduced following overexpression of ARSB activity, and chondroitinase ABC produced similar decline in cell-bound kininogen. Reduction in the cellbound kininogen was associated with increase in secreted bradykinin. In this report, we extend the in vitro findings to in vivo models, and present findings in Dahl salt-sensitive (SS) rats exposed to high (SSH) and low salt (SSL) diets. In the renal tissue of the SSH rats, ARSB activity was significantly less than in the SSL rats, and chondroitin-4-sulfate and total sulfated glycosaminoglycan content were significantly greater. Disaccharide analysis confirmed marked increase in C4S disaccharides in the renal tissue of the SSH rats. In contrast, unsulfated, hyaluronan-derived disaccharides were increased in the rats on the low salt diet. In the SSH rats, with lower ARSB activity and higher C4S levels, cell-bound, high-molecular weight kininogen was greater and urinary bradykinin was lower. ARSB activity in renal tissue and NRK cells declined when exogenous chloride concentration was increased in vitro. The impact of high chloride exposure in vivo on ARSB, chondroitin-4-sulfation, and C4S-kininogen binding provides a mechanism that links dietary salt intake with bradykinin secretion and may be a factor in blood pressure regulation.en_US
dc.description.sponsorshipResearch was supported by VA Merit Awards to R.S. Danziger, M.D. and J.K. Tobacman, M.D. and NIDDK R21HL096031 to Dr. Danzigeren_US
dc.language.isoen_USen_US
dc.publisherSpringer Verlagen_US
dc.subjectbradykininen_US
dc.subjectchondroitinen_US
dc.subjectdisaccharideen_US
dc.subjectsulfataseen_US
dc.subjectsulfateen_US
dc.titleImpact of Salt Exposure on N-Acetylgalactosamine-4-sulfatase (Arylsulfatase B) Activity, Glycosaminoglycans, Kininogen, and Bradykininen_US
dc.typeArticleen_US


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