Show simple item record

dc.contributor.authorKing, Shelby M.
dc.contributor.authorModi, Dimple A.
dc.contributor.authorEddie, Sharon L.
dc.contributor.authorBurdette, Joanna E.
dc.date.accessioned2013-12-06T15:52:17Z
dc.date.available2013-12-06T15:52:17Z
dc.date.issued2013-02
dc.identifier.bibliographicCitationKing, S. M., Modi, D. A., Eddie, S. L. and Burdette, J. E. Insulin and insulin-like growth factor signaling increases proliferation and hyperplasia of the ovarian surface epithelium and decreases follicular integrity through upregulation of the PI3-kinase pathway. Journal of Ovarian Research. 2013. 6. DOI: 10.1186/1757-2215-6-12en_US
dc.identifier.issn1757-2215
dc.identifier.urihttp://hdl.handle.net/10027/10843
dc.description© 2013 King et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2013 by Journal of Ovarian Research, BioMed Central.en_US
dc.description.abstractBackground: The ovarian surface epithelium responds to cytokines and hormonal cues to initiate proliferation and migration following ovulation. Although insulin and IGF are potent proliferative factors for the ovarian surface epithelium and IGF is required for follicle development, increased insulin and IGF activity are correlated with at least two gynecologic conditions: polycystic ovary syndrome and epithelial ovarian cancer. Although insulin and IGF are often components of in vitro culture media, little is known about the effects that these growth factors may have on the ovarian surface epithelium morphology or how signaling in the ovarian surface may affect follicular health and development. Methods: Ovaries from CD1 mice were cultured in alginate hydrogels in the presence or absence of 5 mu g/ml insulin or IGF-I, as well as small molecule inhibitors of IR/IGF1R, PI 3-kinase signaling, or MAPK signaling. Tissues were analyzed by immunohistochemistry for expression of cytokeratin 8 to mark the ovarian surface epithelium, Mullerian inhibiting substance to mark secondary follicles, and BrdU incorporation to assess proliferation. Changes in gene expression in the ovarian surface epithelium in response to insulin or IGF-I were analyzed by transcription array. Extracellular matrix organization was evaluated by expression and localization of collagen IV. Results: Culture of ovarian organoids with insulin or IGF-I resulted in formation of hyperplastic OSE approximately 4-6 cell layers thick with a high rate of proliferation, as well as decreased MIS expression in secondary follicles. Inhibition of the MAPK pathway restored MIS expression reduced by insulin but only partially restored normal OSE growth and morphology. Inhibition of the PI 3-kinase pathway restored MIS expression reduced by IGF-I and restored OSE growth to a single cell layer. Insulin and IGF-I altered organization of collagen IV, which was restored by inhibition of PI 3-kinase signaling. Conclusions: While insulin and IGF are often required for propagation of primary cells, these cytokines may act as potent mitogens to disrupt cell growth, resulting in formation of hyperplastic OSE and decreased follicular integrity as measured by MIS expression and collagen deposition. This may be due partly to altered collagen IV deposition and organization in the ovary in response to insulin and IGF signaling mediated by PI 3-kinaseen_US
dc.description.sponsorshipThe authors would like to thank the Ovarian Cancer Research Fund Liz Tilberis Award L/T/UIC/0.1.2011 and the American Cancer Society Research Scholar Grant from the Illinois Division RSG-12-230-01-TBG for funding this research.en_US
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.subjectInsulinen_US
dc.subjectInsulin-like growth factoren_US
dc.subjectovarian surface epitheliumen_US
dc.subjectovarian follicleen_US
dc.titleInsulin and insulin-like growth factor signaling increases proliferation and hyperplasia of the ovarian surface epithelium and decreases follicular integrity through upregulation of the PI3-kinase pathwayen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record