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    A multicolor panel of TALE-KRAB based transcriptional repressor vectors enabling knockdown of multiple gene targets

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    Date
    2014-12-05
    Author
    Zhang, Z.
    Wu, E.
    Qian, Z.
    Wu, W.S.
    Publisher
    Nature Publishing Group
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    Abstract
    Stable and efficient knockdown of multiple gene targets is highly desirable for dissection of molecular pathways. Because it allows sequence-specific DNA binding, transcription activator-like effector (TALE) offers a new genetic perturbation technique that allows for gene-specific repression. Here, we constructed a multicolor lentiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of multiple gene targets. This platform is fully compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and efficient method for TALE assembly. We showed that this multicolor TALE-KRAB vector system when combined together with bone marrow transplantation could quickly knock down c-kit and PU.1 genes in hematopoietic stem and progenitor cells of recipient mice. Furthermore, our data demonstrated that this platform simultaneously knocked down both c-Kit and PU.1 genes in the same primary cell populations. Together, our results suggest that this multicolor TALE-KRAB vector platform is a promising and versatile tool for knockdown of multiple gene targets and could greatly facilitate dissection of molecular pathways.
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    Type
    Article
    Date available in INDIGO
    2016-01-20T17:02:57Z
    URI
    http://hdl.handle.net/10027/19995
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    • Publications Hematology and Oncology

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