Show simple item record

dc.contributor.authorChen, Dan
dc.contributor.authorChen, Zujian
dc.contributor.authorJin, Yi
dc.contributor.authorDragas, Dragan
dc.contributor.authorZhang, Leitao
dc.contributor.authorAdjei, Barima S.
dc.contributor.authorWang, Anxun
dc.contributor.authorDai, Yang
dc.contributor.authorZhou, Xiaofeng
dc.date.accessioned2016-04-04T18:22:18Z
dc.date.available2016-04-04T18:22:18Z
dc.date.issued2013-12
dc.identifier.bibliographicCitationChen, D., Chen, Z. J., Jin, Y., Dragas, D., Zhang, L. T., Adjei, B. S., Wang, A. X., Dai, Y. and Zhou, X. F. MicroRNA-99 Family Members Suppress Homeobox A1 Expression in Epithelial Cells. PLOS ONE. 2013. 8(12). DOI: 10.1371/journal.pone.0080625.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10027/20357
dc.description.abstractThe miR-99 family is one of the evolutionarily most ancient microRNA families, and it plays a critical role in developmental timing and the maintenance of tissue identity. Recent studies, including reports from our group, suggested that the miR-99 family regulates various physiological processes in adult tissues, such as dermal wound healing, and a number of disease processes, including cancer. By combining 5 independent genome-wide expression profiling experiments, we identified a panel of 266 unique transcripts that were down-regulated in epithelial cells transfected with miR-99 family members. A comprehensive bioinformatics analysis using 12 different sequence-based microRNA target prediction algorithms revealed that 81 out of these 266 down-regulated transcripts are potential direct targets for the miR-99 family. Confirmation experiments and functional analyses were performed to further assess 6 selected miR-99 target genes, including mammalian Target of rapamycin (mTOR), Homeobox A1 (HOXA1), CTD small phosphatase-like (CTDSPL), N-myristoyltransferase 1 (NMT1), Transmembrane protein 30A (TMEM30A), and SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 (SMARCA5). HOXA1 is a known proto-oncogene, and it also plays an important role in embryonic development. The direct targeting of the miR-99 family to two candidate binding sequences located in the HOXA1 mRNA was confirmed using a luciferase reporter gene assay and a ribonucleoprotein-immunoprecipitation (RIP-IP) assay. Ectopic transfection of miR-99 family reduced the expression of HOXA1, which, in consequence, down-regulated the expression of its downstream gene (i.e., Bcl-2) and led to reduced proliferation and cell migration, as well as enhanced apoptosis. In summary, we identified a number of high-confidence miR-99 family target genes, including proto-oncogene HOXA1, which may play an important role in regulating epithelial cell proliferation and migration during physiological disease processes, such as dermal wound healing and tumorigenesis.en_US
dc.description.sponsorshipThis work was supported in part by National Institutes of Health (NIH) PHS grants (CA139596 and CA171436), National Natural Science Foundation of China grants (81072228, 81272953), a grant from Science and Technology Planning Project of Guangdong Province, China (A2010147), a seed grant from CMBOD Oral Cancer Research Program (UIC College of Dentistry), and a Cancer Research Pilot Project grant from University of Illinois Cancer Center. Y.J. is supported by a T32 training grant (DE018381), and D.D. is supported by a R25 grant (DE022675) from NIH/National Institute of Dental and Craniofacial Research. The open access publication fee is supported by the Research Open Access Publishing (ROAAP) fund of the University of Illinois at Chicago. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.language.isoen_USen_US
dc.publisherPLOS ONEen_US
dc.titleMicroRNA-99 Family Members Suppress Homeobox A1 Expression in Epithelial Cellsen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record