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dc.contributor.authorGutka, HJ
dc.contributor.authorWang, Y
dc.contributor.authorFranzblau, SG
dc.contributor.authorMovahedzadeh, F
dc.date.accessioned2016-05-10T16:44:13Z
dc.date.available2016-05-10T16:44:13Z
dc.date.issued2015-09-23
dc.identifier.bibliographicCitationGutka, H. J., Wang, Y., Franzblau, S. G. and Movahedzadeh, F. glpx gene in mycobacterium tuberculosis is required for in vitro gluconeogenic growth and in vivo survival. PLoS One. 2015. 10(9). DOI: 10.1371/journal.pone.0138436.en_US
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10027/20567
dc.descriptionThis is a copy of an article published in the PLoS ONE © 2015 Public Library of Science Publications. © 2015 Gutka et al.en_US
dc.description.abstractSeveral enzymes involved in central carbon metabolism and gluconeogenesis play a critical role in survival and pathogenesis of Mycobacterium tuberculosis (Mtb). The only known functional fructose 1,6-bisphosphatase (FBPase) in Mtb is encoded by the glpX gene and belongs to the Class II sub-family of FBPase. We describe herein the generation of a ΔglpX strain using homologous recombination. Although the growth profile of ΔglpX is comparable to that of wild type Mtb when grown on the standard enrichment media, its growth is dysgonic with individual gluconeogenic substrates such as oleic acid, glycerol and acetate. In mice lung CFU titers of ΔglpX were 2-3 log10 lower than the wild-type Mtb strain. The results indicate that glpX gene encodes a functional FBPase and is essential for both in vitro and in vivo growth and survival of Mtb. Loss of glpX results in significant reduction of FBPase activity but not complete abolition. These findings verify that the glpX encoded FBPase II in Mtb can be a potential target for drug discovery.en_US
dc.description.sponsorshipThis work was supported by the American Lung Association (Grant No. RG-82534-N), and the Chicago Biomedical Consortium with support from The Searle Funds at the Chicago Community Trust.en_US
dc.publisherPublic Library of Scienceen_US
dc.titleglpx Gene in Mycobacterium tuberculosis Is Required for In Vitro Gluconeogenic Growth and In Vivo Survival.en_US
dc.typeArticleen_US


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