Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in Afzelia africana
Date
2016-06-01Author
Jinga, Percy
Palagi, Jason
Ashley, Mary V.
Publisher
Botanical Society of AmericaMetadata
Show full item recordAbstract
Premise of the study:
Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range.
Methods and Results:
Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in A. africana, and eight successfully amplified.
Conclusions:
The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.
Subject
Afzelia africanaAfzelia quanzensis
Fabaceae
Illumina
microsatellite
PAL_FINDER