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    Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in Afzelia africana

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    Development of Microsatellite Loci of POD Mahogany.pdf (523.2Kb)
    Date
    2016-06-01
    Author
    Jinga, Percy
    Palagi, Jason
    Ashley, Mary V.
    Publisher
    Botanical Society of America
    Metadata
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    Abstract
    Premise of the study: Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range. Methods and Results: Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in A. africana, and eight successfully amplified. Conclusions: The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.
    Subject
    Afzelia africana
    Afzelia quanzensis
    Fabaceae
    Illumina
    microsatellite
    PAL_FINDER
    Type
    Article
    Date available in INDIGO
    2016-09-14T19:49:07Z
    URI
    http://hdl.handle.net/10027/21159
    Collections
    • Publications - Biological Sciences

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