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dc.contributor.authorJinga, Percy
dc.contributor.authorPalagi, Jason
dc.contributor.authorAshley, Mary V.
dc.date.accessioned2016-09-14T19:49:07Z
dc.date.available2016-09-14T19:49:07Z
dc.date.issued2016-06-01
dc.identifier.bibliographicCitationJinga, Percy, Jason Palagi, and Mary V. Ashley. "Development of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in A. africana." Applications in Plant Sciences 4, no. 6 (2016). DOI: 10.3732/apps.1600010en_US
dc.identifier.otherESSN: 2168-0450
dc.identifier.urihttp://hdl.handle.net/10027/21159
dc.descriptionThis is a copy of an article published in the Applications in Plant Sciences. © 2016 Jinga et al. https://dx.doi.org/10.3732%2Fapps.1600010en_US
dc.description.abstractPremise of the study: Microsatellite loci were developed for Afzelia quanzensis (Fabaceae) as a first step toward investigating genetic diversity and population structure of the species in its native range. Methods and Results: Illumina shotgun sequencing was used to generate raw sequence reads, which were searched for potential microsatellite loci. A total of 70 potential microsatellite loci were tested for amplification and polymorphism, and 39 successfully amplified. Of the 39 loci that amplified, 12 were polymorphic while 27 were monomorphic. The 12 polymorphic loci were cross-amplified in A. africana, and eight successfully amplified. Conclusions: The 12 polymorphic microsatellite loci can be used for genetic studies of A. quanzensis, which can help determine its conservation status. Eight loci can also be used for genotyping in A. africana.en_US
dc.description.sponsorshipFunding was provided by the University of Illinois at Chicago (UIC) Biological Sciences Department’s Hadley Award, the UIC Office of International Affairs’ Chicago Consular Corps Award, and the Institute of International Education’s Dr. Benjamin L. Van Duuren Grant.en_US
dc.language.isoen_USen_US
dc.publisherBotanical Society of Americaen_US
dc.subjectAfzelia africanaen_US
dc.subjectAfzelia quanzensisen_US
dc.subjectFabaceaeen_US
dc.subjectIlluminaen_US
dc.subjectmicrosatelliteen_US
dc.subjectPAL_FINDERen_US
dc.titleDevelopment of microsatellite loci of pod mahogany, Afzelia quanzensis (Fabaceae), by Illumina shotgun sequencing, and cross-amplification in Afzelia africanaen_US
dc.typeArticleen_US


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