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dc.contributor.authorRehman, Jalees
dc.contributor.authorMarsboom, Glenn
dc.date.accessioned2017-03-03T21:49:03Z
dc.date.available2017-03-03T21:49:03Z
dc.date.issued2016-07-12
dc.identifier.bibliographicCitationMarsboom, G., Zhang, G. F., Pohl-Avila, N., Zhang, Y., Yuan, Y., Kang, H., ... & Rehman, J. (2016). Glutamine metabolism regulates the pluripotency transcription factor OCT4. Cell reports, 16(2), 323-332.en_US
dc.identifier.urihttp://hdl.handle.net/10027/21627
dc.description.abstractThe molecular mechanisms underlying the regulation of pluripotency by cellular metabolism in human embryonic stem cells (hESCs) are not fully understood. We found that high levels of glutamine metabolism are essential to prevent degradation of OCT4, a key transcription factor regulating hESC pluripotency. Glutamine withdrawal depletes the endogenous antioxidant glutathione (GSH), which results in the oxidation of OCT4 cysteine residues required for its DNA binding and enhanced OCT4 degradation. The emergence of the OCT4lo cell population following glutamine withdrawal did not result in greater propensity for cell death. Instead, glutamine withdrawal during vascular differentiation of hESCs generated cells with greater angiogenic capacity, thus indicating that modulating glutamine metabolism enhances the differentiation and functional maturation of cells. These findings demonstrate that the pluripotency transcription factor OCT4 can serve as a metabolic-redox sensor in hESCs and that metabolic cues can act in concert with growth factor signaling to orchestrate stem cell differentiation.en_US
dc.publisherElsevier (Cell Press)en_US
dc.subjectstem cellsen_US
dc.subjectpluripotent stem cellsen_US
dc.subjectembryonic stem cellsen_US
dc.subjectmetabolismen_US
dc.subjectOxidative stressen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectmitochondriaen_US
dc.titleGlutamine Metabolism Regulates the Pluripotency Transcription Factor OCT4en_US
dc.typeArticleen_US


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