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dc.contributor.advisorMerrill, Brad
dc.creatorZhang, Yu
dc.date.accessioned2019-08-06T14:18:03Z
dc.date.available2019-08-06T14:18:03Z
dc.date.created2019-05
dc.date.issued2019-05-28
dc.date.submittedMay 2019
dc.identifier.urihttp://hdl.handle.net/10027/23716
dc.description.abstractThis thesis dissertation examines events which allow embryonic stem cells (ESC) to progress from early or “naïve” pluripotency, a state characterized by propensity for self-renewal, to late or “primed” pluripotency, whence ESC become competent to divide into lineage-specifying cells. This work occurs in two sections. The first section examines the role of the GSK3i-Tcf/Lef axis in the exit from naïve pluripotency. The second section elucidates an epigenetic mechanism by which Tcf7l1 drives exit from naïve pluripotency. Based on conclusions from this work, I propose that Tcf7l1-mediated enhancer decommissioning accommodates the need for pluripotent cells to rapidly alter their response to differentiation signals in the progression towards gastrulation, a likely mechanism by which Wnt/β-catenin signaling controls other stem cell lineage decisions.
dc.format.mimetypeapplication/pdf
dc.subjectDevelopmental Biology, Stem Cells, Embryonic Stem Cells, Wnt signaling, Tcf7l1, Tcf/Lefs, NGS, ATAC-seq, CRISPR
dc.titleThe Role of the GSK3 Inhibition-Tcf7l1 Axis in Facilitating Exit from Naive Pluripotency
dc.typeThesis
thesis.degree.departmentBiochemistry and Molecular Genetics
thesis.degree.grantorUniversity of Illinois at Chicago
thesis.degree.levelDoctoral
thesis.degree.namePhD, Doctor of Philosophy
dc.contributor.committeeMemberBenevolenskaya, Elizaveta
dc.contributor.committeeMemberFrolov, Max
dc.contributor.committeeMemberLau, Lester
dc.contributor.committeeMemberRehman, Jalees
dc.contributor.committeeMemberRaychaudhuri, Pradip
dc.contributor.committeeMemberTyner, Angela
dc.type.materialtext
dc.contributor.chairMerrill, Brad


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