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dc.contributor.authorHoriguchi, Kaori
dc.contributor.authorHanada, Toshihiko
dc.contributor.authorFukui, Yasuhisa
dc.contributor.authorChishti, Athar H.
dc.date.accessioned2009-06-18T18:16:04Z
dc.date.available2009-06-18T18:16:04Z
dc.date.issued2006-07-24
dc.identifier.bibliographicCitationHoriguchi, K., T. Hanada, Y. Fukui, and A. H. Chishti, 2006, Transport of PIP3 by GAKIN, a kinesin-3 family protein, regulates neuronal cell polarity: Journal of Cell Biology, v. 174, no. 3, p. 425-436. http://www.jcb.org.proxy.cc.uic.edu/cgi/content/full/174/3/425en
dc.identifier.issn0021-9525
dc.identifier.otherdoi:10.1083/jcb.200604031
dc.identifier.urihttp://hdl.handle.net/10027/5595
dc.descriptionCopyright: http://www.jcb.org/misc/copyright.pdfen
dc.description.abstractPhosphatidylinositol-(3,4,5)-trisphosphate (PIP3), a product of phosphatidylinositol 3-kinase, is an important second messenger implicated in signal transduction and membrane transport. In hippocampal neurons, the accumulation of PIP3 at the tip of neurite initiates the axon specification and neuronal polarity formation. We show that guanylate kinase - associated kinesin (GAKIN), a kinesin-like motor protein, directly interacts with a PIP3-interacting protein, PIP3BP, and mediates the transport of PIP3-containing vesicles. Recombinant GAKIN and PIP3BP form a complex on synthetic liposomes containing PIP3 and support the motility of the liposomes along microtubules in vitro. In PC12 cells and cultured hippocampal neurons, transport activity of GAKIN contributes to the accumulation of PIP3 at the tip of neurites. In hippocampal neurons, altered accumulation of PIP3 by overexpression of GAKIN constructs led to the loss of the axonally differentiated neurites. Together, these results suggest that, in neurons, the GAKIN-PIP3BP complex transports PIP3 to the neurite ends and regulates neuronal polarity formation.en
dc.language.isoen_USen
dc.publisherRockefeller University Pressen
dc.subjectrhodamine-phosphatidylethanolamineen
dc.subjectphosphatidylcholineen
dc.subjectMAGUK, membrane-associated guanylate kinaseen
dc.subjectFHA, forkhead-associateden
dc.titleTransport of PIP3 by GAKIN, a kinesin-3 family protein, regulates neuronal cell polarityen
dc.typeArticleen


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