Browsing Publications - Pharmaceutical Biotechnology by Title
Now showing items 7-19 of 19
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Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition
(Oxford University Press, 2013-08)Aminoacyl-transfer RNA (tRNA) synthetases (RS) are essential components of the cellular translation machinery and can be exploited for antibiotic discovery. Because cells have many different RS, usually one for each ... -
Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan***not yet published***
(Oxford University Press, 2013-10)A transcriptional attenuation mechanism regulates expression of the bacterial tnaCAB operon. This mechanism requires ribosomal arrest induced by the regulatory nascent TnaC peptide in response to free L-tryptophan (L-Trp). ... -
Ligand efficiency indices for an effective mapping of chemico-biological space: the concept of an atlas-like representation
(Elsevier, 2010-08)We propose a numerical framework that permits an effective Atlas-like representation of Chemico-Biological Space (CBS) based on a series of Cartesian planes mapping the ligands with the corresponding targets connected by ... -
Nascent Peptide in the Ribosome Exit Tunnel Affects Functional Properties of the A-site of the Peptidyl Transferase Center
(Elsevier (Cell Press), 2011-02-04)The ability to monitor the nascent peptide structure and to respond functionally to specific nascent peptide sequences is a fundamental property of the ribosome. An extreme manifestation of such response is nascent ... -
Notes of a protein crystallographer: on the high-resolution structure of the PDB growth rate
(Wiley-Blackwell for the International Union of Crystallography, 2012-05) -
Notes of a protein crystallographer: on the high-resolution structure of the PDB growth rate
(Wiley-Blackwell for the International Union of Crystallography, 2012-05) -
Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides.
(National Academy of Sciences, 2015-10-20)Ketolides are promising new antimicrobials effective against a broad range of Gram-positive pathogens, in part because of the low propensity of these drugs to trigger the expression of resistance genes. A natural ketolide ... -
Structural and biological evaluation of a novel series of benzimidazole inhibitors of Francisella tularensis enoyl-ACP reductase (FabI).
(Elsevier Inc., 2015-03-15)Francisella tularensis, the causative agent of tularemia, presents a significant biological threat and is a Category A priority pathogen due to its potential for weaponization. The bacterial FASII pathway is a viable ... -
Structure of dihydroorotase from Bacillus anthracis at 2.6 A resolution
(International Union of Crystallography, 2010-11)Dihydroorotase (EC 3.5.2.3) catalyzes the reversible cyclization of N-carbamoyl-l-aspartate to l-dihydroorotate in the third step of the pyrimidine-biosynthesis pathway in Bacillus anthracis. A comparison is made between ... -
Structure of dihydroorotase from Bacillus anthracis at 2.6 A resolution
(International Union of Crystallography, 2010-11)Dihydroorotase (EC 3.5.2.3) catalyzes the reversible cyclization of N-carbamoyl-l-aspartate to l-dihydroorotate in the third step of the pyrimidine-biosynthesis pathway in Bacillus anthracis. A comparison is made between ... -
The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics
(National Academy of Sciences, 2010)Crystallographic analysis revealed that the 17-member polyketide antibiotic lankacidin produced by Streptomyces rochei, binds at the peptidyl transferase center of the ubacterial large ribosomal subunit. Biochemical and ... -
Structure of the Francisella tularensis enoyl-acyl carrier protein reductase (FabI) in complex with NAD plus and triclosan
(International Union of Crystallography, 2010-11)Enoyl-acyl carrier protein reductase (FabI) catalyzes the last rate-limiting step in the elongation cycle of the fatty-acid biosynthesis pathway and has been validated as a potential antimicrobial drug target in Francisella ... -
Thermoregulation of Capsule Production by Streptococcus pyogenes
(Public Library of Science, 2012-05)The capsule of Streptococcus pyogenes serves as an adhesin as well as an anti-phagocytic factor by binding to CD44 on keratinocytes of the pharyngeal mucosa and the skin, the main entry sites of the pathogen. We discovered ...