This image is the three-layer overlay image of the differentiated mouse odontoblasts taken from confocal microscope located in UIC medical school. The first layer is the blue color stained nuclei; the second layer is the green color immunostained tublin which represents the cellular skeleton; the third layer is the red color immunostained numb protein. The overlay clearly indicated the physical locations of the numb protein in the cell. Numb has been shown to play critical roles in maintaining the stem cell property in neurons, and we are very excited to identify numb expression in odontoblasts. This discovery may help me to elude the mystery of tooth development.