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dc.contributor.authorPhan, Tung G.
dc.contributor.authorKapusinszky, Beatrix
dc.contributor.authorWang, Chunlin
dc.contributor.authorRose, Robert K.
dc.contributor.authorLipton, Howard L.
dc.contributor.authorDelwart, Eric L.
dc.date.accessioned2012-06-27T21:52:22Z
dc.date.available2012-06-27T21:52:22Z
dc.date.issued2011-09
dc.identifier.bibliographicCitationPhan, T. G., Kapusinszky, B., Wang, C. L., Rose, R. K., Lipton, H. L., & Delwart, E. L. 2011. The Fecal Viral Flora of Wild Rodents. Plos Pathogens, 7(9). DOI: 10.1371/journal.ppat.1002218en
dc.identifier.issn1553-7366
dc.identifier.otherDOI: 10.1371/journal.ppat.1002218
dc.identifier.urihttp://hdl.handle.net/10027/8395
dc.descriptionCopyright © 2011 Phan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. DOI: 10.1371/journal.ppat.1002218en
dc.description.abstractThe frequent interactions of rodents with humans make them a common source of zoonotic infections. To obtain an initial unbiased measure of the viral diversity in the enteric tract of wild rodents we sequenced partially purified, randomly amplified viral RNA and DNA in the feces of 105 wild rodents (mouse, vole, and rat) collected in California and Virginia. We identified in decreasing frequency sequences related to the mammalian viruses families Circoviridae, Picobirnaviridae, Picornaviridae, Astroviridae, Parvoviridae, Papillomaviridae, Adenoviridae, and Coronaviridae. Seventeen small circular DNA genomes containing one or two replicase genes distantly related to the Circoviridae representing several potentially new viral families were characterized. In the Picornaviridae family two new candidate genera as well as a close genetic relative of the human pathogen Aichi virus were characterized. Fragments of the first mouse sapelovirus and picobirnaviruses were identified and the first murine astrovirus genome was characterized. A mouse papillomavirus genome and fragments of a novel adenovirus and adenovirus-associated virus were also sequenced. The next largest fraction of the rodent fecal virome was related to insect viruses of the Densoviridae, Iridoviridae, Polydnaviridae, Dicistroviriade, Bromoviridae, and Virgaviridae families followed by plant virus-related sequences in the Nanoviridae, Geminiviridae, Phycodnaviridae, Secoviridae, Partitiviridae, Tymoviridae, Alphaflexiviridae, and Tombusviridae families reflecting the largely insect and plant rodent diet. Phylogenetic analyses of full and partial viral genomes therefore revealed many previously unreported viral species, genera, and families. The close genetic similarities noted between some rodent and human viruses might reflect past zoonoses. This study increases our understanding of the viral diversity in wild rodents and highlights the large number of still uncharacterized viruses in mammals.en
dc.description.sponsorshipWe acknowledge NHLBI grant R01HL083254 and Blood Systems Research Institute for support to E.L.D and NSF award CNS-0619926 to Bio-X2 cluster at Stanford University for computer resources. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en
dc.language.isoen_USen
dc.publisherPublic Library of Scienceen
dc.titleThe Fecal Viral Flora of Wild Rodentsen
dc.typeArticleen


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