Logo for the University of Illinois at Chicago
    • Login
    View Item 
    •   INDIGO Home
    • Medicine, College of
    • College of Medicine at Chicago
    • Biochemistry and Molecular Genetics, Department of
    • Publications - Biochemistry and Molecular Genetics
    • View Item
    •   INDIGO Home
    • Medicine, College of
    • College of Medicine at Chicago
    • Biochemistry and Molecular Genetics, Department of
    • Publications - Biochemistry and Molecular Genetics
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Regulation of Selective Autophagy Onset by a Ypt/Rab GTPase Module

    Thumbnail
    View/Open
    Segev-PNAS-2012-Indigo (2).pdf (860.0Kb)
    Date
    2012-05
    Author
    Lipatova, Zhanna
    Belogortseva, Natalia
    Zhang, Xiu Qi
    Kim, Jane
    Taussig, David
    Segev, Nava
    Publisher
    National Academy of Sciences
    Metadata
    Show full item record
    Abstract
    The key regulators of intracellular trafficking, Ypt/Rab GTPases, are stimulated by specific upstream activators and, when activated, recruit specific downstream effectors to mediate membrane transport events. The yeast Ypt1 and its human functional homolog hRab1 regulate both endoplasmic reticulum (ER)-to-Golgi transport and autophagy. However, it is not clear whether the mechanism by which these GTPases regulate autophagy depends on their well-documented function in ER-to-Golgi transport. Here, we identify Atg11, the pre-autophagosomal structure (PAS) organizer, as a downstream effector of Ypt1 and show that the Ypt1-Atg11 interaction is required for PAS assembly under normal growth conditions. Moreover, we show that Ypt1 and Atg11 co-localize with Trs85, a Ypt1 activator subunit, and together they regulate selective autophagy. Finally, we show that Ypt1 and Trs85 interact on Atg9-containing membranes, which serve as a source for the membrane component of PAS. Together our results define the first Ypt/Rab module – comprising of activator, GTPase and effector – that orchestrates the onset of selective autophagy, a process vital for cell homeostasis. Furthermore, because Atg11 does not play a role in ER-to-Golgi transport, this is the first demonstration that Ypt/Rabs can regulate two independent membrane transport processes by recruiting process-specific effectors.
    Type
    Article
    Date available in INDIGO
    2012-07-24T02:13:43Z
    URI
    http://hdl.handle.net/10027/8412
    Collections
    • Publications - Biological Sciences
    • Publications - Biochemistry and Molecular Genetics

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us | Send Feedback | Privacy Statement
    Theme by 
    Atmire NV

    Browse

    All of INDIGOCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    DSpace software copyright © 2002-2015  DuraSpace
    Contact Us | Send Feedback | Privacy Statement
    Theme by 
    Atmire NV