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dc.contributor.authorSherwin, J. R. A.
dc.contributor.authorHastings, J. M.
dc.contributor.authorJackson, K. S.
dc.contributor.authorMavrogianis, P. A.
dc.contributor.authorSharkey, A. M.
dc.contributor.authorFazleabas, A. T.
dc.date.accessioned2012-08-21T02:25:07Z
dc.date.available2012-08-21T02:25:07Z
dc.date.issued2010-10
dc.identifier.bibliographicCitationSherwin, J. R. A., Hastings, J. M., Jackson, K. S., Mavrogianis, P. A., Sharkey, A. M., & Fazleabas, A. T. 2010. The Endometrial Response to Chorionic Gonadotropin Is Blunted in a Baboon Model of Endometriosis. Endocrinology, 151(10): 4982-4993. doi: 10.1210/en.2010-0275en
dc.identifier.issn0013-7227
dc.identifier.issndoi: 10.1210/en.2010-0275
dc.identifier.urihttp://hdl.handle.net/10027/8597
dc.description© 2010 by The Endocrine Society. The original version is available through Endocrine Society at doi: 10.1210/en.2010-0275en
dc.description.abstractEndometriosis-associated infertility has a multifactorial etiology. We tested the hypothesis that the endometrial response to the early embryonic signal, human chorionic gonadotropin (hCG), alters over time in a nonhuman primate model of endometriosis. Animals with experimental or spontaneous endometriosis were treated with hCG (30 IU/d), from d 6 after ovulation for 5 d, via an oviductal cannula. Microarray analysis of endometrial transcripts from baboons treated with hCG at 3 and 6 months of disease (n6) identified 22 and 165 genes, respectively, whose levels differed more than 2-fold compared with disease-free (DF) animals treated with hCG (P 0.01). Quantitative RT-PCR confirmed abnormal responses of known hCG-regulated genes. APOA1, SFRP4, and PAPPA, which are normally down-regulated by hCG were up-regulated by hCG in animals with endometriosis. In contrast, the ability of hCG to induce SERPINA3 was lost. Immunohistochemistry demonstrated dysregulation of C3 and superoxide dismutase 2 proteins. We demonstrate that this abnormal response to hCG persists for up to 15 months after disease induction and that the nature of the abnormal response changes as the disease progresses. Immunohistochemistry showed that this aberrant gene expression was not a consequence of altered LH/choriogonadotropin receptor distribution in the endometrium of animals with endometriosis. We have shown that endometriosis induces complex changes in the response of eutopic endometrium to hCG, which may prevent the acquisition of the full endometrial molecular repertoire necessary for decidualization and tolerance of the fetal allograft. This may in part explain endometriosis-associated implantation failure. (Endocrinology 151: 4982–4993, 2010)en
dc.description.sponsorshipThis work was supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development/National Institutes of Health through cooperative agreement (U54 HD 40093, to A.T.F.) as part of the Specialized Cooperative Centers Program in Reproduction and Infertility Research.en
dc.language.isoen_USen
dc.publisherEndocrine Societyen
dc.titleThe Endometrial Response to Chorionic Gonadotropin Is Blunted in a Baboon Model of Endometriosisen
dc.typeArticleen


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