Show simple item record

dc.contributor.authorSong, Min
dc.contributor.authorJin, JingJi
dc.contributor.authorLim, Jeong-Eun
dc.contributor.authorKou, Jinghong
dc.contributor.authorPattanayak, Abhinandan
dc.contributor.authorRehman, Jamaal A
dc.contributor.authorKim, Hong-Duck
dc.contributor.authorTahara, Kazuki
dc.contributor.authorLalonde, Robert
dc.contributor.authorFukuchi, Ken-ichiro
dc.date.accessioned2012-08-21T02:42:40Z
dc.date.available2012-08-21T02:42:40Z
dc.date.issued2011-08
dc.identifier.bibliographicCitationSong, M., Jin, J., Lim, J. E., Kou, J., Pattanayak, A., Rehman, J. A., Kim, H. D., Tahara, K., Lalonde, R., & Fukuchi, K. 2011. TLR4 mutation reduces microglial activation, increases Aβ deposits and exacerbates cognitive deficits in a mouse model of Alzheimer's disease. Journal of Neuroinflammation, 8: 92. DOI: 10.1186/1742-2094-8-92en
dc.identifier.issn1742-2094
dc.identifier.otherDOI: 10.1186/1742-2094-8-92
dc.identifier.urihttp://hdl.handle.net/10027/8601
dc.description© 2011 Song et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. DOI: 10.1186/1742-2094-8-92en
dc.description.abstractBackground: Amyloid plaques, a pathological hallmark of Alzheimer’s disease (AD), are accompanied by activated microglia. The role of activated microglia in the pathogenesis of AD remains controversial: either clearing Aβ deposits by phagocytosis or releasing proinflammatory cytokines and cytotoxic substances. Microglia can be activated via toll-like receptors (TLRs), a class of pattern-recognition receptors in the innate immune system. We previously demonstrated that an AD mouse model homozygous for a loss-of-function mutation of TLR4 had increases in Aβ deposits and buffer-soluble Aβ in the brain as compared with a TLR4 wild-type AD mouse model at 14-16 months of age. However, it is unknown if TLR4 signaling is involved in initiation of Aβ deposition as well as activation and recruitment of microglia at the early stage of AD. Here, we investigated the role of TLR4 signaling and microglial activation in early stages using 5-month-old AD mouse models when Aβ deposits start. Methods: Microglial activation and amyloid deposition in the brain were determined by immunohistochemistry in the AD models. Levels of cerebral soluble Aβ were determined by ELISA. mRNA levels of cytokines and chemokines in the brain and Aβ-stimulated monocytes were quantified by real-time PCR. Cognitive functions were assessed by the Morris water maze. Results: While no difference was found in cerebral Aβ load between AD mouse models at 5 months with and without TLR4 mutation, microglial activation in a TLR4 mutant AD model (TLR4M Tg) was less than that in a TLR4 wild-type AD model (TLR4W Tg). At 9 months, TLR4M Tg mice had increased Aβ deposition and soluble Aβ42 in the brain, which were associated with decrements in cognitive functions and expression levels of IL-1β, CCL3, and CCL4 in the hippocampus compared to TLR4W Tg mice. TLR4 mutation diminished Aβ-induced IL-1β, CCL3, and CCL4 expression in monocytes. Conclusion: This is the first demonstration of TLR4-dependent activation of microglia at the early stage of β-amyloidosis. Our results indicate that TLR4 is not involved in the initiation of Aβ deposition and that, as Aβ deposits start, microglia are activated via TLR4 signaling to reduce Aβ deposits and preserve cognitive functions from Aβ-mediated neurotoxicity.en
dc.description.sponsorshipThis work was supported in part by grants from the National Institutes of Health (AG030399, AG031979, AG029818 and EY018478) and the Alzheimer’s Association (IIRG-07-59494).en
dc.language.isoen_USen
dc.publisherBioMed Centralen
dc.titleTLR4 mutation reduces microglial activation, increases Aβ deposits and exacerbates cognitive deficits in a mouse model of Alzheimer’s diseaseen
dc.typeArticleen


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record