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dc.contributor.advisorMiller, Lawrenceen_US
dc.contributor.authorGahlaut, Nivritien_US
dc.date.accessioned2012-12-10T16:35:30Z
dc.date.available2012-12-10T16:35:30Z
dc.date.created2012-05en_US
dc.date.issued2012-12-10
dc.date.submitted2012-05en_US
dc.identifier.urihttp://hdl.handle.net/10027/9109
dc.description.abstractThis dissertation presents the results of two projects that shared the common goal of improving contrast in cellular imaging; one, instrumental and the other, probe-based. The instrumental approach is focused on the development, optimization and characterization of a Time resolved luminescence (TRL) microscope for lanthanide imaging that employs a light-emitting diode (LED, λem = 365 nm) for pulsed epi-illumination and an intensified charge-coupled device (ICCD) camera for gated, widefield detection. Instrument performance was evaluated in terms of short-lifetime fluorescence background rejection, photon collection efficiency, image contrast, and signal-to-noise ratio (SNR). The performance analyses show that TRL microscopy is sufficiently sensitive and precise to allow high-resolution, quantitative imaging of lanthanide luminescence in living cells under physiologically relevant experimental conditions. The probe-based approach entails the synthesis and characterization of monovalent, ligand-functionalized, dendrimer-encapsulated, gold nanoparticles that were designed to label specific proteins for correlative luminescence and electron microscopy (CLEM). Specifically, the synthetic strategies for functionalizing cystamine core G4 and G5 PAMAM dendrimers monovalently with a protein targeting ligand (TMP and biotin) and with multiple fluorophores as well as templating gold nanoparticle growth are presented.en_US
dc.language.isoenen_US
dc.rightsCopyright 2012 Nivriti Gahlauten_US
dc.subjecttime-resolved microscopyen_US
dc.subjectprotein-targeted dendrimersen_US
dc.subjectgold nanoparticlesen
dc.titleImproving contrast in biological imaging: time-resolved microscopy and protein-targeted dendrimersen_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineChemistryen_US
thesis.degree.grantorUniversity of Illinois at Chicagoen_US
thesis.degree.levelDoctoralen_US
thesis.degree.namePhD, Doctor of Philosophyen_US
dc.type.genrethesisen_US
dc.contributor.committeeMemberWo-Mei Fung, Leslieen_US
dc.contributor.committeeMemberCho, Wonwhoen_US
dc.contributor.committeeMemberSnee, Prestonen_US
dc.contributor.committeeMemberHong, Seungpyoen_US
dc.type.materialtexten_US


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